BBa_J52011

BBa_J52011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J52011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monika Ciglic
Date created: 2006-10-13 11:00:00
Date modified: 2015-08-31 03:54:04

dnMyD88-linker-Rluc



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J52011_sequence (Version 1)

Description

dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.

Notes

dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.

Source

-

Sequence Annotation Location Component / Role(s)
dnMyD88
linker
Rluc
dnMyD88-linker-Rluc
1,417
418,435
436,1371
1,1371
feature/cds CDS
feature/protein CDS
tag feature/tag
engineered_region feature/BioBrick
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J52011/1