BBa_J52011

BBa_J52011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J52011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Monika Ciglic
Date created: 2006-10-13 11:00:00
Date modified: 2015-08-31 03:54:04

dnMyD88-linker-Rluc



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_J52011_sequence (Version 1)

    Description

    dnMyD88 is a dominant negative form of protein MyD88 (an important protein in signaling cascade through TLR4 receptor), which was our main target for modifying the signaling path. dnMyD88 is linked to Rluc (which acts as a reporter) through six aminoacid long protein linker.

    Notes

    dnMyD88-linker-Rluc was made by using PCR ligation technique. This part is cloned in BioBrick vector pSB1AK3.

    Source

    -

    Sequence Annotation Location Component / Role(s)
    dnMyD88
    linker
    Rluc
    dnMyD88-linker-Rluc
    		1,417
    418,435
    436,1371
    1,1371
    		feature/cds CDS
    feature/protein CDS
    tag feature/tag
    engineered_region feature/BioBrick

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J52011/1
     

    Attachments

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