Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jelka Pohar
Date created: 2006-10-14 11:00:00
Date modified: 2015-08-31 03:54:04
green fluorescent protein derived from A. victoria GFP (SwissProt: P42212) with PEST191 tag
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_J52028_sequence (Version 1) |
Description
GFP-linker-PEST191 is a part composed of GFP (as a reporter) linked with PEST191 through six aminoacid long protein linker. PEST191 is a sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h.Notes
GFP-linker-PEST191 is made by PCR overlap extension technique.Source
GFP-PEST191-
| Sequence Annotation | Location | Component / Role(s) |
| GFP linker PEST191 (degradation flag) GFP-linker-PEST191 start codon stop codon | 1,717 718,735 736,1221 1,1221 1,3 1219,1221 | feature/cds CDS feature/protein CDS tag feature/tag feature/BioBrick engineered_region start_codon feature/start stop_codon feature/stop |