BBa_J58011

BBa_J58011 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_J58011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Caterina Mata Garcia

Date created: 2006-10-22 11:00:00

Date modified: 2015-08-31 03:14:48

Promoter which is activated by cI and CRP, using a transcription logic function type AND

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_J58011_sequence (Version 1)

Description

Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding site for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. Thus, both proteins interact directly with RNA polymerase.

Notes

We take the lambda Prm promoter sequence from the Registry, and we add the CRP binding site upstream of that promoter. The centered position of the cI operator is -42, and for the CRP is -93,5.

References:

JK Joung, DM Koepp, A Hochschild. Synergistic activation of transcription by bacteriophage lambda cI protein and E. coli cAMP receptor protein. Science (1994), 265, 1863-1866.

Source

The sources of this promoter are, on the one hand, from the bacteriophage lambda, and, on the other hand, from the E. coli. We take the lambda Prm promoter (modified to be activated but not repressed by cI, part ref. BBa_I12007), and the binding site region for CRP from E. coli.

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J58011/1

Member of these Collections

• iGEM Parts Registry