CmR

BBa_J61000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J61000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-07-30 11:00:00
Date modified: 2015-08-31 02:02:59

chloramphenicol resistance cassette



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_J61000_sequence (Version 1)

    Description

    Chloramphenicol resistance gene including its native promoter and ribosome binding site. Confers resistance to 25 ug/mL chloramphenicol.

    {pSB1A2-Bca1016}

    Notes

    N/A

    Source


    PCR ca1016F/R on pKD3 (934 bp, EcoRI/SpeI)

    Sub into pSB1A2-I13522 (EcoRI/SpeI)

    Products are Bca1015 and Bca1016


    ca1016F Forward EcoRI to biobrick CAT of pKD3

    gacttgaattcgcggccgcttctagaggaataggaacttcatttaaatg

    ca1016R Reverse SpeI to biobrick CAT of pKD3

    ccgctactagtggcgcgcctacctgtgacgg



    Sequence Annotation Location Component / Role(s)
    CmR
    		30,687
    		CDS feature/cds

    igem#experience
    Works
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J61000/1
     

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