BBa_J61015

BBa_J61015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J61015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2006-08-10 11:00:00

Date modified: 2015-08-31 02:02:59

Biobrick converter for NotI-part-BamHI

Details

• Types: DnaRegion
• Roles: Other; sequence_feature
• Sequences: BBa_J61015_sequence (Version 1)

Description

Inserting a NotI/BamHI fragment into the PspOMI/BglII sites of J61015 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.

Notes

N/A

Source

PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

Sub into pSB1A2 (EcoRI/SpeI)

Products are:

Bca1007 NotI/BamHI transfer

Bca1008 BamHI/EcoRI transfer

Bca1009 NotI/EcoRI transfer

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ca1007F Forward EcoRI for 5' PspOMI NotI converter

ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg

ca1007R Reverse SpeI for 3' BglII BamHI converter

ccgctactagtagatcttataaacgcagaaaggcccac

ca1008F Forward EcoRI for 5' BglII BamHI converter

ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg

ca1009R Reverse SpeI for 3' MfeI EcoRI converter

ccgctactagtcaattgtataaacgcagaaaggcccac

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J61015/1

Member of these Collections

• iGEM Parts Registry