BBa_J61016

BBa_J61016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J61016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-08-10 11:00:00
Date modified: 2015-08-31 02:02:59

Biobrick converter for BamHI-part-EcoRI



    • Details

    Types
    DnaRegion

    Roles
    Other

    sequence_feature

    Sequences BBa_J61016_sequence (Version 1)

    Description

    Inserting a BamHI/EcoRI fragment into the BglII/MfeI sites of J61015 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.

    Notes

    N/A

    Source


    PCR ca1007F/R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

    PCR ca1008F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

    PCR ca1007F/ca1009R on pSB1A2-J01064 (972 bp, EcoRI/SpeI)

    Sub into pSB1A2 (EcoRI/SpeI)

    Products are:

    Bca1007 NotI/BamHI transfer

    Bca1008 BamHI/EcoRI transfer

    Bca1009 NotI/EcoRI transfer


    ca1007F Forward EcoRI for 5' PspOMI NotI converter

    ctctggaattcgcggccgcttctagagggccctccctatcagtgatagagattg

    ca1007R Reverse SpeI for 3' BglII BamHI converter

    ccgctactagtagatcttataaacgcagaaaggcccac

    ca1008F Forward EcoRI for 5' BglII BamHI converter

    ctctggaattcgcggccgcttctagaagatcttccctatcagtgatagagattg

    ca1009R Reverse SpeI for 3' MfeI EcoRI converter

    ccgctactagtcaattgtataaacgcagaaaggcccac


    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J61016/1
     

    Attachments

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