Types | DnaRegion
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Roles | sequence_feature
DNA
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Sequences | BBa_J61020_sequence (Version 1)
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Description
Site for recombination by flp recombinase. Genes flanked by FRT sites (oriented in the same direction) in the genome can be excised with the introduction of flp recombinase-expressing helper plasmid pCP20. Note that the original FRT sequence contained an XbaI site that has been removed with a point mutation for compatibility with standard assembly. See Datsenko and Wanner for details of its use in markerless knockouts and knockins.
Notes
N/A
Source
Extend ca1010F/R (73 bp, EcoRI/SpeI)
Sub into pSB1A2-I13522 (EcoRI/SpeI)
Product is pSB1A2-Bca1010
ca1010F Forward (universal biobrick) EcoRI for FRT
GGACTgaattcgcggccgcttctagag
ca1010R Reverse SpeI oligo for FRT
cctatactagtagaagttcctattctctaAaaagtataggaacttcctctagaagcggccgcg