BBa_J61032

BBa_J61032 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J61032
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2006-11-10 12:00:00
Date modified: 2015-08-31 02:02:59

Alkaline phosphatase [phoA]



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_J61032_sequence (Version 1)

    Description

    Alkaline phosphatase coding sequence (no rbs, promoter, or terminator present) from Citrobacter. This bacterium's phoA was chosen due simply to the absense of Biobrick restriction sites.

    Notes

    N/A

    Source

    pre-pcr ca1033F/ca1032R on Citrobacter gen (1500 bp)
    PCR ca1032F/R on above PCR (1500 bp, XbaI/EcoRI)
    Sub into pSB1A2-Bca1008 (XbaI/MfeI, 2077+935, large)
    Product is pSB1A2-Bca1032

    ----
    ca1032F Forward oligo to biobrick phoA of Citrobacter
    GGTTAtctagaTGTTTTTTATTTTAACTGTATTTATACATGGAG
    ca1032R Reverse oligo to biobrick phoA of Citrobacter
    cagtaGAATTCccagaggcggcaTTACTTCAG
    ca1033F Forward constant oligo to clone phoA
    ttatcatcgctccgcatcctg

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