BBa_J61035

BBa_J61035 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J61035

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2006-11-21 12:00:00

Date modified: 2015-08-31 02:02:59

EcoRI-GenR-XbaI

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_J61035_sequence (Version 1)

Description

Selectable marker inserted between EcoRI and XbaI restriction sites. The sequence inluded describes plasmid pSB1A2-b0034 (ribosome binding site part) with this cassette inserted between EcoRI and XbaI. This part facilitates the transfer of small parts during biobrick assembly. Because it is fairly large in size, EcoRI/SpeI digests of the plasmid can be readily gel purified. Because it contains a selectable marker, cloning products containing the cassette can be selected on gentamicin plates.

Notes

N/A

Source

Complicated

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
beta-lactamase (ampR) promoter	1859,1887	feature/promoter, promoter
R6K origin	2090,2469	feature/dna, sequence_feature
Gentamycin resistance	2535,3068	CDS, feature/cds
ColE1 origin	180,862	feature/dna, sequence_feature
beta-lactamase (ampicillin resistance marker)	957,1817	CDS, feature/cds
R6Kg origin	2091,2474	feature/dna, sequence_feature
pBR322 origin	183,802	feature/dna, sequence_feature
BioBrick suffix	1,21	feature/dna, sequence_feature
EcoRI	2058,2063	sequence_feature, feature/dna
NotI	2514,2521	feature/dna, sequence_feature
XbaI	3533,3538	sequence_feature, feature/dna

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J61035/1

Member of these Collections

• //plasmid
• iGEM Parts Registry