Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Caroline Ajo-Franklin
Date created: 2006-10-13 11:00:00
Date modified: 2015-08-31 01:56:26
Low copy protein fusion vector (Silver lab standard)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_J63009_sequence (Version 1) |
Description
Silver Lab alias V0002. This is a modified biobrick vector (fusion brick vector) that contains the specified EcoRI, SpeI, XbaI, and PstI sites. The only difference is that the part sequence immediately follows the XbaI site and immediately precedes the SpeI site. This change allows in-frame fusions of protein coding regions to be made using standard biobricks assembly methods.Notes
If the part starts with the nucleotides tc, then that part-containing plasmid must be grow in a DamI- strain to avoid methylation (and inhibition of digestion) at the XbaI site.Source
based on pUC19 plasmid| Sequence Annotation | Location | Component / Role(s) |
| modified BioBrick suffix RNAII transcript rep pMB1 replication origin RNAI transcript beta-lactamase (bla gene) bla gene bla(P3) promoter -10 sequence bla(P3) promoter -35 sequence modified BioBrick prefix | 1,20 197,749 182,796 639,746 956,1816 1824,1828 1860,1865 1881,1886 2057,2077 | feature/dna sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS ribosome_entry_site feature/rbs promoter feature/promoter promoter feature/promoter feature/dna sequence_feature |