Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Widmaier
Date created: 2008-01-15 12:00:00
Date modified: 2015-08-31 01:56:26
3x FLAG affinity tag
| Types | DnaRegion |
| Roles | Tag tag |
| Sequences | BBa_J64005_sequence (Version 1) |
Description
A 3 repeat FLAG (DYKDDDDK) antigen epitope tag used for marking proteins of interest. Proteins carrying this tag can be easily detected using commercial western blotting reagents.Notes
This sequence was designed as part of an oligonucleotide primer on each strand then annealed and ligated into the vector.Source
Synthetic DNA from Oligonucleotide synthesis| Sequence Annotation | Location | Component / Role(s) |
| 3x FLAG Tag STOP Codon XbaI Restriction Site | 1,72 73,75 76,81 | feature/protein CDS stop_codon feature/stop feature/misc sequence_feature |