BBa_J64982

BBa_J64982 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J64982
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sai Duriseti
Date created: 2007-03-25 11:00:00
Date modified: 2015-05-08 01:08:18

OmpR-P strong binding, regulatory region for Team Challenge 03-2007



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_J64982_sequence (Version 1)

Description

Contains the F1 consensus site from the OmpF regulator. Contains a LacI repressor consensus binding site between the -35 and -10 portions of a T7 core promoter region and a sigma 70 region, and a TetR consensus binding site upstream of the -35 site, and the sigma 70 region

Notes

The weaker binding repressor binds upstream of the core promoter (upstream of -35), while the stronger binds inbetween the -35 and -10.

Source

Information for creation of this construct came from the following sorces:
Yoshida et al. JBC 2006
Frank et al. JMB 1997
Rong et al. PNAS 1998
Merabet et al. Biochemistry 1995
Su et al. JBC 1998
Kedracka-Krok et al. JPC 1999
Kleinschmidt et al. Biochemistry 1988
Butler et al. JBC 1982
Jorgensen et al. JBC 1991
Hillen et al. JMB 1984
Head et al. JMB 1998
Huang et al. JMB 1996

Sequence Annotation Location Component / Role(s)
TetR
1,19
non_covalent_binding_site feature/binding
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J64982/1