Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_J64982_sequence (Version 1)
|
Description
Contains the F1 consensus site from the OmpF regulator. Contains a LacI repressor consensus binding site between the -35 and -10 portions of a T7 core promoter region and a sigma 70 region, and a TetR consensus binding site upstream of the -35 site, and the sigma 70 region
Notes
The weaker binding repressor binds upstream of the core promoter (upstream of -35), while the stronger binds inbetween the -35 and -10.
Source
Information for creation of this construct came from the following sorces:
Yoshida et al. JBC 2006
Frank et al. JMB 1997
Rong et al. PNAS 1998
Merabet et al. Biochemistry 1995
Su et al. JBC 1998
Kedracka-Krok et al. JPC 1999
Kleinschmidt et al. Biochemistry 1988
Butler et al. JBC 1982
Jorgensen et al. JBC 1991
Hillen et al. JMB 1984
Head et al. JMB 1998
Huang et al. JMB 1996