Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2008-10-19 11:00:00
Date modified: 2015-05-08 01:08:20
Promoter for tetM gene, from pBOT1 plasmid, pAMbeta1
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_J70025_sequence (Version 1) |
Description
Upstream promoter for tetM gene; contains a short peptide leader, regulatory sequences which are poorly understood. Use in combination with J70024.Notes
Alignment of the promoter with the coding region, preservation of the RBS sites.Source
pBOT1 plasmid PCR. pBOT1 from J. Renaudin. Original sequence is part of pAMbeta1 tetracycline resistance plasmid.| Sequence Annotation | Location | Component / Role(s) |
| RBS term stem_loop stem_loop stem_loop rbs -10 -35 +1 leader peptide | 339,345 319,330 273,320 254,281 242,272 237,240 68,73 45,50 80,80 250,336 | ribosome_entry_site feature/rbs stem_loop feature/stem_loop feature/stem_loop stem_loop feature/stem_loop stem_loop stem_loop feature/stem_loop ribosome_entry_site feature/rbs feature/promoter promoter promoter feature/promoter feature/misc sequence_feature feature/cds CDS |