Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2008-11-04 12:00:00
Date modified: 2015-05-08 01:08:20
Mesoplasma florum plasmid origin
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_J70027_sequence (Version 1) |
Description
This part is a cassette containing the PE gene from the pSci2 plasmid of Spiroplasma citri. It is reported to convey replication ability for the group 1 spiroplasmas. The part contains the replication origin, the promoter, and gene for the PE protein.Notes
No native restriction sites. Added biobrick ends.The gene for PE contains no CGG codons.
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGtaattataaaaacaccttattttac,J00023-F2
GTTTCTTCCTGCAGCGGCCGCTACTAGTAttgggagtgcggggtttggggtgc,J00023-R
The start of J70027 matches the origin start in the pSci1 plasmid.
Source
Genbank AJ969070| Sequence Annotation | Location | Component / Role(s) |
| J70023-F2 dnaA binding dnaA binding imperfect repeat dnaA binding imperfect repeat dnaA binding PE RBS iteron iteron Low GC region J70023-R | 1,25 152,160 218,226 282,293 296,304 403,413 587,595 654,1307 640,644 1305,1315 1325,1335 1470,1646 1678,1701 | feature/primer_binding primer_binding_site sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature CDS feature/cds ribosome_entry_site feature/rbs sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/primer_binding primer_binding_site |