Types | DnaRegion
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Roles | Temporary
sequence_feature
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Sequences | BBa_J70315_sequence (Version 1)
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Description
Built using S03621 using the following primers:
forward (60 bp) cut with XbaI
gccgcttctagagGCCTGAACGGCCTTTACaaagaggagaaatactagatggtgagcaag
reverse (take reverse complement before making-- 60 bp) cut with PsrI
TCTTCCTGCAGCGGCCGCTACTAGTAAAGGCCGTTCagagcgttcaccgacaaacaacag
actual reverse
ctgttgtttgtcggtgaacgctctGAACGGCCTTTACTAGTAGCGGCCGCTGCAGGAAGA
Notes
testing part, should be red during expression, should cut in invariant regions of scars, prefix, suffix
Source
S03621