Types | DnaRegion
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Roles | sequence_feature
Other
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Sequences | BBa_J70488_sequence (Version 1)
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Description
These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-GFP to (Promoter X)-(RBS level Y)-GFP.
Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)
(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)
Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}
{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}
Forward oligo:
g {rbs master sequence} atg c gtaaa
Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} atg c)
Notes
none
Source
designed