Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2009-09-12 11:00:00
Date modified: 2015-05-08 01:08:23
pWV01 broad host range plasmid origin and rep protein
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_J70500_sequence (Version 1) |
Description
pWV01 broad host range plasmid origin and rep protein.pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.
Notes
Avoided a single Sau3AI site near the claI site, to eliminate Sau3AI sites in the final plasmid assembly.Source
PCR of the plasmid pMG, kind gift from Prof. Jan Kok. This plasmid contains the ClaI fragment of the pWV01 plasmid.| Sequence Annotation | Location | Component / Role(s) |
| orfB orfC (CopG) repA orfD ir-I ir-II ir-III ir-IV (terminator) Promoter P1 direct repeat 1 direct repeat 2 direct repeat 3 | 335,544 585,746 813,1511 1508,1657 50,181 228,250 334,382 1656,1697 522,550 456,466 467,478 479,490 | feature/cds CDS CDS feature/cds CDS feature/cds CDS feature/cds feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |