Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2009-12-08 12:00:00
Date modified: 2015-05-08 01:08:24
gfp RBS, {0,5
15,10} family member - B0035 simulator (reverse oligo)
| Types | DnaRegion |
| Roles | Temporary sequence_feature |
| Sequences | BBa_J70541_sequence (Version 1) |
Description
These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;15,10})-GFP to (Promoter X)-(RBS level Y)-GFP.Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)
(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)
Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}
{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}
Forward oligo:
g {rbs master sequence} atg c gtaaa
Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} atg c)
Notes
BioScaffold insertSource
These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;15,10})-GFP to (Promoter X)-(RBS level Y)-GFP.Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)
(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)
Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}
{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}
Forward oligo:
g {rbs master sequence} atg c gtaaa
Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} atg c)