Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-03-29 11:00:00
Date modified: 2015-05-08 01:08:24
luxAB Fusion gene, Xenorhabdus luminescens
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_J70565_sequence (Version 1) |
Description
This part includes a fusion of the coding sequences for the luxA and luxB genes of the organism Xenorhabdus luminescens, along with luxA's native ribosomal binding site.The coding regions provided by K216008,luciferase LuxAB of Xenorhabdus luminescens, were used in the fusion gene. Via a standard fusion PCR reaction luxA's stop codon was removed and the intervening nucleotides were replaced with a 10 amino Gly-Ser linker. In addition, the nonfunctional SpeI cut site in K216008 was fixed so that the part could be assembled with others in the future.
LuxAB codes for a dimer that in the presence of decanal, produces light. This decanal can either be provided in-vitro with n-Decanal, or in-vivo by the luxCDABE cassete (which will produce tetradecanal).
Notes
A 10 amino Gly-Ser linker was chosen to maintain functionality of the dimer, but this was fairly arbitrary and the length of the intervening linker may need to be changed.Source
Amplified from part K216008.| Sequence Annotation | Location | Component / Role(s) |
| rbs luxAB coding sequence luxAB start Gly-Ser linker luxAB stop | 15,20 27,2123 27,29 1104,1142 2121,2123 | feature/rbs ribosome_entry_site feature/cds CDS feature/start start_codon sequence_feature feature/dna feature/stop stop_codon |