Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2010-04-29 11:00:00
Date modified: 2015-05-08 01:08:24
J70570(AK) +J70568(AT) into (AC)
| Types | DnaRegion |
| Roles | Temporary sequence_feature |
| Sequences | BBa_J70572_sequence (Version 1) |
Description
intermediate circuitNotes
original J70570 and J70568 had bad restriction enzyme sites in themSource
designeddigest J70562-AK with BsaXI and insert this to add a C-terminal tag
forward (J70562-fi): aaGGATCCCACCACCACCACCACCACTAATAAtactagtagcggc
reverse (J70562-ri): gctactagtaTTATTAGTGGTGGTGGTGGTGGTGGGATCCtttgt
look for green colonies, send six for sequencing, colony pcr-- think about doing his-tag purification later
| Access | Instance | Definition |
| public public | BBa_J70570 BBa_J70568 | BBa_J70570 BBa_J70568 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J70570 BBa_J70568 | 1,912 921,1969 | BBa_J70570 BBa_J70568 |