Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Julie Norville
Date created: 2010-05-17 11:00:00
Date modified: 2015-05-08 01:08:24
pyrrolysyl-tRNA synthetase (PylRS)???tRNAPyl (pylT)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_J70584_sequence (Version 1) |
Description
Methanosarcina mazei Go1, complete genomepylS pyrolysyl-tRNA synthetase [ Methanosarcina mazei Go1 ]
Notes
noneSource
web,4.2 Construction of pBK-PylRS
The plasmid pBK-PylRS was derived from pBK-JYRS.[7] The gene of
Methanosarcina mazei PylRS was PCR amplified from Methanosarcina mazei
genomic DNA which was purchased from ATCC. Two restriction sites, Nde1 at the 5???
end and Pst1 at the 3??? end, were introduced in the PCR product which was
subsequently digested and cloned into pBK-JYRS to replace JYRS.