BBa_J70594

BBa_J70594 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70594
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-06-17 11:00:00
Date modified: 2015-05-08 01:08:25

RFC12 TAATAA Tail Domain



    • Details

    Types
    DnaRegion

    Roles
    polypeptide_domain

    Protein_Domain

    Sequences BBa_J70594_sequence (Version 1)

    Description

    A RFC12 compatible part that simply codes for two stop codons. This part does not have any degradation tag.

    Notes

    Made with synthetic oligos:
    5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3'
    5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3'


    Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

    Source

    Common Knowledge

    Sequence Annotation Location Component / Role(s)
    stop
    		1,5
    		stop_codon feature/stop

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J70594/1
     

    Attachments

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