BBa_J70595

BBa_J70595 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70595
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-06-22 11:00:00
Date modified: 2015-05-08 01:08:25

RFC12 Green Fluorescent Protein



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_J70595_sequence (Version 1)

Description

This is a RFC12 compatible part based off of BBa_E0040.

Notes

Amplified via colony PCR directly from the BBa_E0040 cell stock.

Forward Primer:
5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3'
Reverse Primer:
3' CACATGGCATGGATGAACTATACAAA 5'
so
5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3'

Source

BBa_E0040

Sequence Annotation Location Component / Role(s)
GFP
1,710
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J70595/1