Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-07-15 11:00:00
Date modified: 2015-05-08 01:08:25
RFC12 7x His Tag Head Domain
| Types | DnaRegion |
| Roles | tag Tag |
| Sequences | BBa_J70609_sequence (Version 1) |
Description
This is a 7x His tag which is commonly used to tag proteins for purification purposes. It is an RFC12 compatible head domain mean for N-terminal fusions.Notes
Constructed using oligos:AATTC GCGGCCGC T ACTAGT ATGAAACACCATCACCATCACCATCAC GCTAGC A GCGGCCG CTGCA, His-F
G CGGCCGC T GCTAGC GTGATGGTGATGGTGATGGTGTTTCAT ACTAGT A GCGGCCGC G
Source
Registry part BBa_T2001.| Sequence Annotation | Location | Component / Role(s) |
| start codon Preferred AAA +2 codon 7x His Tag | 1,3 4,6 7,25 | start_codon feature/start start_codon feature/start feature/protein CDS |