Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-08-04 11:00:00
Date modified: 2015-05-08 01:08:25
RFC12 Maltose Binding Protein Internal Domain [malE]
| Types | DnaRegion |
| Roles | tag Tag |
| Sequences | BBa_J70620_sequence (Version 1) |
Description
This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein.MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
Notes
Primers:| Sequence Annotation | Location | Component / Role(s) |
| MBP Asparagine Spacer | 1,1093 1094,1138 | feature/cds CDS tag feature/tag |