Submit
Login or Register
View
BBa_J70621
BBa_J70621
Version 1
Component
Source:
http://parts.igem.org/Part:BBa_J70621
Generated By:
https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joseph Lynch
Date created: 2010-08-07 11:00:00
Date modified: 2015-05-08 01:08:25
RFC12 7x His Tag Tail Domain
Download
Download SBOL File
Download COMBINE Archive
Download GenBank
Download GFF3
Download FASTA
Search
Find Uses
Find Twins
Send
Send to Benchling
Send to ICE
Back
Details
Types
DnaRegion
Roles
Tag
tag
Sequences
BBa_J70621_sequence (Version 1)
Description
This is a RFC12 compatible version of
BBa_T2000
. It is a 7x his tag meant for C-terminal fusions.
Notes
Oligos used:
AATTC GCGGCCGC T ACTAGT CACCATCACCATCACCATCACTAATAA GCTAGC A GCGGCCG CTGCA, HisT-F
GCGGCCGCTGCTAGC TTATTAGTGATGGTGATGGTGATGGTG ACTAGTAGCGGCCGCG, HisT-R
Source
Synthesized
Sequence Annotations
Sequence Annotation
Location
Component
/ Role(s)
His Tag
Stop codons
1,20
21,25
feature/tag
tag
stop_codon
feature/stop
Other Properties
igem#sampleStatus
Not in stock
igem#status
Unavailable
synbiohub#ownedBy
user/james
synbiohub#ownedBy
user/myers
synbiohub#topLevel
BBa_J70621/1
Member of these Collections
iGEM Parts Registry
Attachments
Sequence Visualization
92087b5d-1ff4-4bf3-9828-45ba64b0ea4f
Component Sankey
b13a6263-ace9-4d0f-9b82-f6cc1cd98299
Component Bar
b0756c4a-9070-4511-9c37-eacd85c89426