BBa_J70624

BBa_J70624 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J70624

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Joseph Lynch

Date created: 2010-08-07 11:00:00

Date modified: 2015-05-08 01:08:25

RFC12 FLAG Tag Head Domain

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_J70624_sequence (Version 1)

Description

This is a RFC12 compatible FLAG tag meant for N-terminal fusions. See the RFC10 equivalent, T2004 for more information.

Notes

Oligos used:
AATTC GCGGCCGC T ACTAGT ATGAAAGACTATAAGGATGACGATGACAAA GCTAGC A GCGGCCG CTGCA, FLAG-F
GCGGCCGCTGCTAGCTTTGTCATCGTCATCCTTATAGTCTTTCATACTAGTAGCGGCCGCG, FLAG-R

Source

Synthesized

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
FLAG tag	7,28	tag, feature/tag
ATG Start	1,3	start_codon, feature/start
Preferred +2 codon	4,6	start_codon, feature/start

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J70624/1

Member of these Collections

• iGEM Parts Registry