Types | DnaRegion
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Roles | engineered_region
Generator
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Sequences | BBa_J70636_sequence (Version 1)
|
Description
pheS gene from the phenylalanine tRNA charging complex in M. florum, preparing for mutation for use with p-chlorophenylalanine counter selection (Kast, pKSS).
Notes
none.
Source
PCR from M. florum genomic DNA. Primers:
- ttccgaattcgcggccgcttctagag ctctgttattggtatagtta,pheS-mf-F2
- ttccactagtatgttctacctactctcc,pheS-mf-R