BBa_J70636

BBa_J70636 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J70636
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tom Knight
Date created: 2010-11-01 12:00:00
Date modified: 2015-05-08 01:08:25

pheS gene from M. florum



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_J70636_sequence (Version 1)

Description

pheS gene from the phenylalanine tRNA charging complex in M. florum, preparing for mutation for use with p-chlorophenylalanine counter selection (Kast, pKSS).

Notes

none.

Source

PCR from M. florum genomic DNA. Primers:
  • ttccgaattcgcggccgcttctagag ctctgttattggtatagtta,pheS-mf-F2
  • ttccactagtatgttctacctactctcc,pheS-mf-R


Sequence Annotation Location Component / Role(s)
pheS
94,1146
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J70636/1