BBa_J72006

BBa_J72006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_J72006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Anderson

Date created: 2008-07-15 11:00:00

Date modified: 2015-05-08 01:08:26

{M.BamHI-2} BamHI methyltransferase in BBb

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_J72006_sequence (Version 1)

Description

This part is the ORF for the BamHI methyltransferase. The enzyme it encodes methylates the sequence GGATCC and makes that sequence uncleavable by BamHI restriction enzyme. In house, this part is referred to as pBca9145-Bca1149 {M.BamHI-2}.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

PCR ca1149F/ca1142R on Bacillus amyloliquefaciens genomic DNA  (BglII/XhoI)

Sub into pBca9145-Bca1144#5                                    (BglII/XhoI, L)

Product is pBca9145-Bca1149



ca1149F	Forward BglII for M.BamHI

ggtgtAGATCTatgcgAttCttCtctgtCtttgacattg

ca1142R	Reverse Biobricking of M.bamHI

gcaaaCTCGAGttaGGATCCTCATTCcAGACTTAATGAAATTTGGTC

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• rdf-schema#seeAlso: P23941
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_J72006/1

Member of these Collections

• iGEM Parts Registry