BBa_J72010

BBa_J72010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2008-07-15 11:00:00
Date modified: 2015-05-08 01:08:26

{lacZ>} lacZ basic part with no stop in BBb



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_J72010_sequence (Version 1)

    Description

    lacZ gene encoding beta-galactosidase

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Notes

    N/A

    Source


    
PCR ca1133F/ca1134R on MG1655 genomic DNA  (3065, BglII/XhoI)
    
Sub into pBca9145-Bca1089                  (BglII/XhoI)
    
Product is pBca9145-Bca1133
    


    
ca1133F	LacZ eXtreme with start no stop
    
ctctgAGATCTATGACCATGATTACGGATTC
    
ca1134R	Removing EcoRI in lacZ
    
CTGGTAATGGTAGCGACCGGCGCTCAGCTGaAATTCCGCCGATAC


    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J72010/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (57f00336)