Types | DnaRegion
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Roles | Coding
CDS
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Sequences | BBa_J72012_sequence (Version 1)
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Description
BglII methyltransferase open reading frame basic part. Methylates the sequence AGATCT.
This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:
BBb Standard Description Page
Notes
N/A
Source
PCR ca1143F/ca1143R on Bacillus globigii gen. (BglII/XhoI)
Sub into pBca9145-Bca1144#5 (BglII/XhoI,L)
Product is pBca9145-Bca1143 {M.BglII)
ca1143F Forward Biobricking of M.bglII
ctctgAGATCTatgagtgaagatcaatataaac
ca1143R Forward Biobricking of M.bglII
gcaaaCTCGAGttaGGATCCttattgttcattttgaactt