BBa_J72021

BBa_J72021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll42} in BBb



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_J72021_sequence (Version 1)

Description

{rbs_at_10000.HSV} N terminal HSV tag: RBS with an HSV tag.



This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll42 {rbs_at_10000.HSV>}



Wobble mll42/mll43 (105, EcoRI/BamHI
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll42 {rbs_at_10000.HSV.}


mll42 Forward wobble for {rbs_at_10000.HSV>} (Bmll42)
CCATAGAATTCATGAGATCTCGAACACCCATATCAATATTAAAGGGAAGGTAAGCATGCAGCCGGAG
mll43 Reverse wobble for {rbs_at_10000.HSV>} (Bmll42)
CGTTAGGATCCGCAGTCTTCCGGGTCTTCTGGTGCGAGCTCCGGCTGCATGCTTACC


Sequence Annotation Location Component / Role(s)
HSV tag
RBS at 10000
44,80
6,40
feature/cds CDS
feature/rbs ribosome_entry_site
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_J72021/1