BBa_J72036

BBa_J72036 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J72036
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jennifer Brophy
Date created: 2010-07-14 11:00:00
Date modified: 2015-05-08 01:08:26

{Bmll66} in BBb



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_J72036_sequence (Version 1)

    Description

    {VSV!} C terminal VSV tag.

    This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Notes

    N/A

    Source

    Overlap Extension PCR (Wobble Construction)

    Wobble construction of Bmll66 {

    
Wobble mll66/mll67                (67, EcoRI/BamHI
    
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
    
Product is pBca9523-Bmll66         {


    
mll66    Forward wobble for {
CCATAGAATTCATGAGATCTTACACCGATATAGAGATGAACAGGCTGGG
    
mll67    Reverse wobble for {
CGTTAGGATCCttaCTTTCCCAGCCTGTTCATCTCTATA


    Sequence Annotation Location Component / Role(s)
    VSV tag
    		6,39
    		CDS feature/cds

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_J72036/1
     

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