| Types | DnaRegion
|
| Roles | Translational_Unit
engineered_region
|
| Sequences | BBa_J90001_sequence (Version 1)
|
Description
This blue chromoprotein (BCP) generator is useful as an insert for assembly plasmids as it provides a simple way to screen for correctly assembled plasmids (blue colonies contain the original plasmid, not the desired construct). Unlike fluorescent proteins, chromoproteins are not very useful for quantitative measurements. Therefore, we recommend using this BCP generator as a screenable insert instead of reporter cassettes that use fluorescent proteins such as GFP or RFP. The promoter driving BCP expression is BBa_R0010 which can be repressed by lacI and CAP. Therefore, this reporter is most useful for screening colonies on LB-agar plates (or cultures in liquid LB) and should not be used on a plasmid that also expressed lacI.
Notes
None, except for acknowledging the limitations of the promoter BBa_R0010.
Source
This is a composite part.
