View

BBa_K076007

BBa_K076007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K076007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM2008 Team Princeton

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:08:32

pLV-TRE-Ngn1-linker-EYFP-Ubc-Bla

Back

Details

Types	DnaRegion
Roles	plasmid Plasmid
Sequences	BBa_K076007_sequence (Version 1)

Description

This plasmid only contains the fluorescently-labeled Ngn1, which enables the location of this neuronal transcription factor to be determined within the cell.

Notes

This plasmid was constructed by digesting pLV-TRE-Sox17-Ubc-Bla with SfiI to excise TRE-Sox17, and inserting the PCR SOEd SfiI-Ngn1-linker-EYFP-SfiI.

Source

This plasmid was constructed by PCR SOEing.

Components

Access	Instance	Definition
public public public public	BBa_K076001 BBa_K076002 BBa_K076003 BBa_K076004	BBa_K076001 BBa_K076002 BBa_K076003 BBa_K076004

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K076001 BBa_K076002 BBa_K076003 BBa_K076004	1,10859 10866,11597 11606,11659 11666,12382	BBa_K076001 BBa_K076002 BBa_K076003 BBa_K076004

Other Properties

igem#experience	Works
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K076007/1