Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM2008 Princeton team
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:08:32
pLV-TRE-Lbx1-T2A-GLRA1-P2A-D5R-Ubc-Bla
| Types | DnaRegion |
| Roles | plasmid Plasmid |
| Sequences | BBa_K076013_sequence (Version 1) |
Description
This plasmid allows for the simultaneous production of Lbx1 [produces GABAergic neurons], GLRA1 [a glycine receptor], and D5R [a subunit of a particular dopamine receptor]. This makes the neurons able to respond to both a dopamine and a glycine input, and in turn produce a GABA output.Notes
This plasmid was constructed by PCR SOEing.Source
This plasmid was constructed by digesting pLV-TRE-Sox17-Ubc-Bla with SfiI to excise TRE-Sox17, and inserting the PCR SOEd SfiI-Lbx1-T2A-GLRA1-P2A-D5R-SfiI.| Access | Instance | Definition |
| public public public public public public | BBa_K076001 BBa_K076008 BBa_K076009 BBa_K076010 BBa_K076011 BBa_K076012 | BBa_K076001 BBa_K076008 BBa_K076009 BBa_K076010 BBa_K076011 BBa_K076012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K076001 BBa_K076008 BBa_K076009 BBa_K076010 BBa_K076011 BBa_K076012 | 1,10859 10866,12299 12306,13148 13155,14501 14510,14563 14572,14628 | BBa_K076001 BBa_K076008 BBa_K076009 BBa_K076010 BBa_K076011 BBa_K076012 |