BBa_K076015

BBa_K076015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K076015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM2008 Princeton team

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:08:32

pLV-TRE-Mash1-IRES2-mKate

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_K076015_sequence (Version 1)

Description

This plasmid encourages differentiation of embryonic stem cells into neuronal precursors and marks the cells with the red-fluorescent mKate.

Notes

EGFP could not be taken out due to restriction cut site considerations. Mash1-IRES-mKate therefore had to be constructed using PCR SOEing and inserted whole.

Source

pLV-TRE-Mash1-IRES-EGFP was digested with EcoRI and BsrGI to remove Mash1-IRES-EGFP. Mash1-IRES-mKate was inserted into this vector, having digested the ends with EcoRI and BsiWI (BsrGI and BsiWI have compatible overhangs for ligation).

Components

Access	Instance	Definition
public	BBa_I745000	BBa_I745000
public	BBa_K076014	BBa_K076014
public	BBa_K076006	BBa_K076006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I745000	1,9519	BBa_I745000
BBa_K076014	9526,10221	BBa_K076014
BBa_K076006	10228,10941	BBa_K076006

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K076015/1

Member of these Collections

• iGEM Parts Registry