BBa_K076018

BBa_K076018 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K076018

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM2008 Princeton team

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:08:32

pLV-TRE-D5R-Ubc-Bleo

Details

• Types: DnaRegion
• Roles: Plasmid; plasmid
• Sequences: BBa_K076018_sequence (Version 1)

Description

This plasmid allows bleomycin-selected cells to express the D5R dopamine receptor subunit upon induction with doxycycline.

Notes

This plasmid was constructed by PCR SOEing.

Source

PLV-TRE-IRES2-EGFP [BBa_I745000] was digested with EcoRI and BsrGI, which allowed the insertion of the PCR SOEd EcoRI-D5R-Ubc-Bleo-BsiWI.

Components

Access	Instance	Definition
public	BBa_I745000	BBa_I745000
public	BBa_K076008	BBa_K076008
public	BBa_K076017	BBa_K076017
public	BBa_K076016	BBa_K076016

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I745000	1,9519	BBa_I745000
BBa_K076008	9526,10959	BBa_K076008
BBa_K076017	10968,12186	BBa_K076017
BBa_K076016	12193,12567	BBa_K076016

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K076018/1

Member of these Collections

• iGEM Parts Registry