BBa_K078000

BBa_K078000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K078000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sally.Wang (Jingyi Wang)

Date created: 2008-07-13 11:00:00

Date modified: 2015-05-08 01:08:33

A fragment that encodes an enzyme involed in dioxin degradation

Details

• Types: DnaRegion
• Roles: Coding; CDS

Description

It is 2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase, which catalyze the meta cleavage reaction, the second step, in the process of 4-chlorodibenzofuran degradation, from 2,2',3-Trihydroxybiphenyl to 2-Hydroxy-6-oxo-6-(2-hydroxyphenyl)-hexa-2,4-dienoate. The size is relatively small, 885bp.
CDS: 1 tcaatgcgcc ggcaactgca actcgtggcc ccaaatgctc ggtgcgtcat agaatccaac
61 atgccaactt tccaaatctc caatcacctg gccaccaaag cccaactcaa ctgcaaaacc
121 agccggtgcc atcatgtaga aggagtagac cttatcattg gagtgctggc caattgtcat
181 gaggacatca tagcccaagc gcttgaccgc tgcgaacgcg cgatcaacct gctctttgct
241 cttggcttct agcatcagat gatgaaccat acctggctta agaccgagcg ccggtgccga
301 aagtgcaacg gtgtgatgac gctggttgca gtgaaggaag aagatgtcgg cttcaggcca
361 agttacccga tcactgagtc cgaagcccag aacatcagtg aaaaaagatt gagtcttcgc
421 ccgatcagcc acgatcacta cgaggtgccc caagccgaag atgccgttcg cttcttcacc
481 acagacgaat agagactcga gcacagggtc ggtgggaaac gacgattcga catacagttc
541 atgggtaaag ccatcgttat cggcgaactg tcgaacttgg gcggccgcgc gatctgcggc
601 cagttccgca tcagccgtta cgaccgaata acatgccttc tccacctgtt ccgctatcgc
661 cgccagatca ctagggtcta cattccaacc gatcgccgca aggcggtttg cggggccagg
721 atcgagcctg atacggaaag gtcggccatc aaggcgaaca agagcgcgtc cgccttcatc
781 acgctccacc acagcaccga tgatatcttg gtatacgact accatttgct ccagaacatc
841 agccctacat tcaaaaataa ggtagccaag ttgttttact gacat

Notes

We plan to use it to construct the pathway of dioxin degradation.

Source

It comes from genome of the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1, which is provided by Dr. Rolf Wittich, Zaidin Experimental Station CSIC, Spain.

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K078000/1

Member of these Collections

• iGEM Parts Registry