BBa_K078004

BBa_K078004 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K078004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sally.Wang (Jingyi Wang)

Date created: 2008-10-14 11:00:00

Date modified: 2015-05-08 01:08:33

2，
2???，
3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati

Details

• Types: DnaRegion
• Roles: CDS; Coding

Description

2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase. It is the second step meta cleavage enzyme in dixon degradation pathway.

This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except Not1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.

Notes

We use this part to construct the degradation pathway of dioxin.
This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except Not1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h.

Source

It is from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1.
Type: genomic DNA

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K078004/1

Member of these Collections

• iGEM Parts Registry