BBa_K078008

BBa_K078008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K078008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sally.Wang (Jingyi Wang)

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:08:33

bphC codes 2,3-Dihydroxybiphenyl-1,2-dioxygenase

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K078008_sequence (Version 1)

Description

bphC codes 2,3-Dihydroxybiphenyl-1,2-dioxygenase that is the third step enzyme in PCBs degradation pathway.

This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used. The vector is the standard vector, pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+pstI.Digest in NEBBuffer EcoRI + BSA at 37??C for about 3h.

Notes

We use this part to construct the degradation pathway of PCBs.

Source

From the PCBs-degrading bacterium Burkholderia xenovorans LB400 chromosome 3.
Type: genomic DNA

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
bphC	1,898	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K078008/1

Member of these Collections

• //function/degradation
• iGEM Parts Registry