BBa_K091101

BBa_K091101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin Feeney

Date created: 2008-05-28 11:00:00

Date modified: 2015-05-08 01:08:37

pTet_Lac hybrid promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K091101_sequence (Version 1)

Description

This promoter is repressed by tetR and lacI; it contains two binding sites for TetR and one LacI binding site. this part is an AND-gate since transcription occurs only in the presence of both ATC and IPTG.

Notes

Also designed by Pallavi Penumetcha and Robert Cool.

Source

This part consists of the R0040 tetR promoter with the addition of the R0010 lacI binding site.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
tetR binding site 1	1,19	feature/binding, non_covalent_binding_site
tetR binding site 2	26,44	feature/binding, non_covalent_binding_site
-35	20,25	sequence_feature, feature/misc
-10	43,48	sequence_feature, feature/misc
lacI binding site	49,83	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091101/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/multiple
• //regulation/negative
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry