BBa_K091107

BBa_K091107 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K091107
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xiao Zhu
Date created: 2008-06-18 11:00:00
Date modified: 2015-05-08 01:08:37

pLux/cI Hybrid Promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K091107_sequence (Version 1)

Description

This hybrid promoter is designed to respond to activation by LuxR when it is bound to AHL and to repression by the phage lambda cI repressor. The repression needs to win over activation.

Notes

This part is based on the idea of replacing the region of the Lux promoter downstream of the -35 with the OR2 cI half-operator, added LuxR-10 region. Based on what we have found up to now, promoters which are constructed just by inserting OR1/2 downstream of Lux are all very leaky.

Source

this part comes from LuxR promoter and cI lambda promoter.

Sequence Annotation Location Component / Role(s)
Lux Box
Lux -35
cI OR2
Lux -10
1,20
20,25
26,43
44,49
operator feature/operator
feature/promoter promoter
feature/operator operator
promoter feature/promoter
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K091107/1