Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Pallavi Penumetcha
Date created: 2008-07-08 11:00:00
Date modified: 2015-05-08 01:08:38
LacI_X86 protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K091124_sequence (Version 1) |
Description
This is a variation of the LacI wild-type protein. It is characterized by a missense mutation in the 61st codon in which serine is mutated to leucine. This mutation causes a 10,000 fold increase in binding affinity of this protein for the pLac promoter.Notes
Because there are more than one codons that code for leucine, we chose CTG, because it was the most common leucine codon found in the rest of the LacI wild-type gene.Source
The LacI wild-type gene sequence came from Ecogene.com| Sequence Annotation | Location | Component / Role(s) |
| serine->leucine | 181,183 | sequence_feature feature/misc |