BBa_K091127

BBa_K091127 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091127

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Pallavi Penumetcha

Date created: 2008-07-09 11:00:00

Date modified: 2015-05-08 01:08:38

LacI_I12_X86

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K091127_sequence (Version 1)

Description

This protein is a variation of LacI protein and has missense mutations at its third codon (proline->tyrosine) and 61st codon (serine->leucine). This causes a 10,000 fold increase in its binding affinity for the pLac promoter.

Notes

See parts K091125 and K091123 for design considerations

Source

The basic sequence for the LacI gene came from Ecogene.com.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
proline->tyrosine	7,9	feature/misc, sequence_feature
serine->leucine	181,183	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091127/1

Member of these Collections

• iGEM Parts Registry