BBa_K091143

BBa_K091143 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091143

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Xiao Zhu

Date created: 2008-07-14 11:00:00

Date modified: 2015-05-08 01:08:38

pLas/cI Hybrid Promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K091143_sequence (Version 1)

Description

this hybrid promoter is designed to be consecutively off.It combines with plasR(R0079) and lambda cI OR1(see C0051).Adding P-AI-1 sould activate the promoter, while repressed by lambda cI.

Notes

"CI binding of the OR region. It binds OR1 with highest affinity, OR2 with next highest, and OR3 with lowest"(http://www.nonanone.com/qw/files/Biology%204450/test2_lecture8,9.txt)

Source

.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	140,145	feature/promoter, promoter
-35	117,122	promoter, feature/promoter
cI OR1	147,164	operator, feature/operator
plasR OP1	46,63	feature/operator, operator
plasR OP2	106,126	operator, feature/operator

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091143/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //function/cellsignalling/lasr
• //promoter
• //regulation/multiple
• //regulation/negative
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry