BBa_K091156

BBa_K091156 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K091156

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Erin Feeney

Date created: 2008-07-24 11:00:00

Date modified: 2015-05-08 01:08:38

pLux

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K091156_sequence (Version 1)

Description

The pLux' promoter is a modified version of BBa_R0062 containing the -35 region TTTAAT in place of TTTACG. Transcriptional activation is expected to occur in the presence of LuxR and AI-1, just as with R0062. The modified -35 region was designed to serve as a control for the evaluation of BBa_K091156, the pLux+ Las- promoter.

Notes

This promoter is identical to pLux+ Las- with the exception of the Las box's absence

Source

This part was adapted from BBa_R0062.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-35 Region	20,25	feature/misc, sequence_feature
-10 Region	42,47	feature/misc, sequence_feature
LuxR	1,20	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K091156/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //function/cellsignalling/luxr
• //promoter
• //regulation/positive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry