Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: EPF-Lausanne Team 2008
Date created: 2008-10-18 11:00:00
Date modified: 2015-05-08 01:08:40
TetR cds with RBS and Terminator + pTetR, RFP with RBS + LuxI with RBS and terminator
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K092700_sequence (Version 1) |
Description
Combination of the following parts : BBa_K092000, BBa_K092300 and BBa_K092400:The TetR sequence (with a ribosome binding site and terminator)[BBa_K09200] is bound to the part containing the promoter of TetR and the red fluorescent protein (RFP) with a ribosome binding site. A luxI part (with RBS and terminator) is added at the end of the cds of the RFP. As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.
| Access | Instance | Definition |
| public public | BBa_K092600 BBa_K092400 | BBa_K092600 LuxI |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K092600 BBa_K092400 | 1,1634 1643,2414 | BBa_K092600 LuxI |