BBa_K093015

BBa_K093015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K093015
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Heil
Date created: 2009-04-19 11:00:00
Date modified: 2015-05-08 01:08:40

phi C31 integrase



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K093015_sequence (Version 1)

    Description

    This ORF codes for the steptomyces phage phi C31 integrase, which catalyzes a site specific recombination between phiC31 attB and attP sequences.

    Notes

    Removed EcoRI site via site directed mutagenesis, replaced non standard start codon, and replaced non standard stop codon.

    Source

    PCR amplified from pHS62

    Thorpe, H. M. & Smith, M. C. (1998). In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci U S A 95, 5505-5510.

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K093015/1
     

    Attachments

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