Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ellen Martin
Date created: 2008-07-27 11:00:00
Date modified: 2015-05-08 01:08:41
Construct with TetR/p22mnt promoter, RBS, RFP, Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K101006_sequence (Version 1) |
Description
TetR is a promoter. p22mnt is a promoter. RBS is the ribosomal binding site. RFP is the red fluorescent protein, which is a reporter gene. The terminator will terminate transcription.Notes
Majority of design considerations were dealt with during the design of the dually repressed promoter (K101000). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.Source
Construct is composed of: TetR promoter (R0040), p22mnt promoter (R0073), RBS (B0032), RFP (J06504), and Terminator (B1006).| Access | Instance | Definition |
| public public public public | BBa_K101000 BBa_B0032 BBa_J06504 BBa_B1006 | BBa_K101000 BBa_B0032 mCherry BBa_B1006 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K101000 BBa_B0032 BBa_J06504 BBa_B1006 | 1,61 70,82 89,802 811,849 | BBa_K101000 BBa_B0032 mCherry BBa_B1006 |